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Rapid screen for UTI

Works on the basis that certain bacteria reduce nitrate to nitrite

Detects about 70% of Clinically significant UTI bacteria

All bacteria don't posses the enzyme reductase necessary to break nitrate down (most gram - do and most gram + don't)

First morning specimen is best

Requires 100,000 organisms per mL to get a positive

Positive is any degree of uniform pink color

Urine has to be in the bladder with the bacteria approximately 4 hrs to convert bitrate to nitrite

Useful in detecting cystitis, pyelonephritis, monitoring antibiotic therapy and as a screening test

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At what temperature should the urine be prior to testing with the reagent strip method?

Test the sample as soon as possible. If testing cannot be performed within an hour after voiding, refrigerate the specimen immediately at 2–8oC (38-42oF) and let it return to room temperature before testing.

Why does a refrigerated urine sample must be returned to room temperature before chemical testing by reagent strips?

SPECIMEN COLLECTION AND PREPARATION voiding, refrigerate the specimen immediately and let it return to room temperature before testing. temperature may result in microbial proliferation with resultant changes in pH. A shift to alkaline pH may cause false positive results with the protein test area.

How long can a urine specimen remain at room temperature before it must be refrigerated quizlet?

For the most accurate results, urine specimens should be kept refrigerated and processed within 2 hours of collection. Hematuria is the presence of intact red blood cells (RBCs) in urine.

When performing a reagent strip test for protein false positive results may be obtained in the presence of?

(2) Protein: False positive results may be obtained with highly buffered or alkaline urines. Contamination of the urine specimen with quaternary ammonium compounds (from some antiseptics and detergents) or with cleansers containing chlorhexidine may also produce false positive results.