Media Credits The audio, illustrations, photos, and videos are credited beneath the media asset, except for promotional images, which generally link to another page that contains the media credit. The Rights Holder for media is the person or group credited. Show
Director Tyson Brown, National Geographic Society Author National Geographic Society Production Managers Gina Borgia, National Geographic Society Jeanna Sullivan, National Geographic Society Program Specialists Sarah Appleton, National Geographic Society, National Geographic Society Margot Willis, National Geographic Society otherSEEING IS BELIEVING Light MicroscopesMicroscopes that use visible light to view the microscopic world are broadly categorized as "light microscopes". Compound Microscopes are a type of light microscope that uses a series of 2 lenses in order to achieve higher magnifying power than simple microscopes having only one lens (also known as "dissecting microscopes".) When using a compound microscope, specimen must be placed on a transparent microscope slide and covered with a transparent cover slip in order to view. The specimen is then placed on the stage of the microscope over the light source to allow light to pass through the specimen. The illuminated specimen is viewed through 2 magnifying lenses; 1) the magnifying lens of the objective located on the rotating nose-piece which is positioned directly over the specimen, and 2) the magnifying lens located in the eye-pieces. The eyepieces have a fixed magnification of 10X. The rotating nosepiece will have an array of objectives ranging from 4X up to perhaps 100X, depending on the microscope. Each objective is clearly marked indicating its magnification power.
Field of ViewThe field of view DECREASES as you INCREASE magnification. You should notice that your DEPTH OF
FOCUS decreases as magnification INCREASES. This means that with a relatively small movement of the fine focus knob, you could be out of the focal range. For looking at cells, you will notice you will see fewer layers of cells at higher magnifications. Inversion
The compound microscope allows us to magnify objects up to 1000 times (with a 100X objective). We can calculate the magnification we are observing with the microscope by multiplying the magnification power of the objective, by the magnification power of the eyepieces. The magnification of the eyepieces is always 10X. There fore, If you are using the 40X objective, the object you are viewing under the microscope would be magnified 400 times more than with the naked eye. If we do the math, we see that 10X times 40X equals 400X. Compound microscopes are good for viewing up to 1 micrometer, or 100 nanometers (nm).
HOW TO USE THE COMPOUND MICROSCOPEI. HANDLING Before using the compound microscope, it is important to know how to handle and care for it. When transporting the microscope, it is very important to make sure that you have a good grip on the scope and carry it with 2 hands at all times. One hand should be placed on the base of the scope while the other should be placed on the body (see diagram). The scopes are not necessarily heavy, but their center of mass is at the base, making it unstable if supporting by one hand alone. You must also be sure that any cords are wrapped up and secured before supporting so as to not trip over the cord or create a hazard for others around you to possibly trip and fall. II. INSTRUCTIONSSkipping or overlooking any steps could result in very costly damage to the objectives, eyepieces or other microscopic parts. Please take care to read instruction first, before doing each and every step.
1) Place microscope gently on the counter at your workstation.
IV. INCREASING MAGNIFICATION
Can You Identify These Parts of The Microscope?
PUTTING AWAY SCOPES1) Turn off POWER switch. PARFOCAL Field of View
The field of view DECREASES as you INCREASE magnification.
What happens to an image under a microscope?The image of an object is magnified through at least one lens in the microscope. This lens bends light toward the eye and makes an object appear larger than it actually is.
What part of the microscope focuses the image of the specimen?Condenser Lens: The purpose of the condenser lens is to focus the light onto the specimen. Condenser lenses are most useful at the highest powers (400x and above).
What happens to an image when viewed under a compound microscope?The images seen under the microscope are inverted. The relative direction of movement is also reversed, i.e., moving the stage to the left will be viewed as moving to the right. The images seen under the microscope are inverted.
|