In this exercise you will become familiar with a bright field microscope that you'll be using throughout the semester. Show
Bright Field Microscope ComponentsA: Eyepiece or ocular lenses. Magnify 10X B: Nosepiece C: Specimen holder D: Condenser. Focuses the light onto the specimen E. Aperture Iris Diaphragm. Controls the amount of light that enters the objective F: Field iris diaphragm. Controls the amount of light leaving the source. G: Light Source H: X-axis control knob. Moves specimen left and right I: Oil-immersion objective lens. Magnifies 100X J: High-dry objective lens. Magnifies 40X K: Low-power objective lens: Magnifies 10X L: Power switch M: Knob to control light intensity N: Coarse adjustment focusing knob. Makes large changes in focus O: Fine adjustment focusing knob. Makes small changes in focus P: Y-axis control knob. Moves specimen up and down
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Back to Top What is the term for a microscope that stays in focus as the objective lens is changed?Parfocal (parfocality)
- a term used describing the property of a microscope where the subject stays in focus when the objective lenses are changed.
What term describes objective lenses that remain in focus when one is focused?Parfocal means that the microscope is binocular. B. Parfocal means that when one objective lens is in focus, then the other objectives will also be in focus.
Which type of objective are you going to use in focusing a specimen?Focusing with theLow Power Objective. Low power objectives cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens. This objective is useful for aligning the microscope. The power for the low objective is 10X.
What is focusing on the specimen under a microscope?Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing.
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