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I. PurposeThis document provides guidelines for providing post-operative care and for recognizing and alleviating signs of pain and distress in rodents. II. ScopeThis applies to all individuals providing post-operative care and observations of post- operative rodents. III. GuidanceThe NRC Guide for the Care and Use of Laboratory Animals: states that an integral component of medical care is prevention or alleviation of pain associated with procedural or surgical protocols. Pain is a stressor and can lead to unacceptable levels of stress and distress in animals. Fundamental to the relief of pain is the ability to recognize its clinical signs in specific species, as species vary in their response to pain. The NIH guidelines state that in general, unless the contrary is known or established, it should be considered that procedures that cause pain in humans may also cause pain in other animals. IV. ResponsibilitiesThe surgeon and research staff are responsible for:
V. Post-Operative Care of RodentsProvision of non-pharmacologic post-operative care, including nursing support, can assist with control of pain and promote well-being of the animal. Abnormalities noted during postoperative care, should be reported to the veterinarian.
VI. DocumentationA written survival surgery/post-operative care log must be maintained in the animal housing room for the first 7 days after surgery and be available for review by the AV. The IACUC may also request copies of the documents. The log should include the following information:
VII. Recognizing Signs of Pain and Distress in Rodents
IACUC Approval Date: 08/18/2021 Review Date: 08/18/2021 Issue Date: 08/30/2021 Why is monitoring animals after a procedure beneficial?Systematically Monitoring for Pain and Distress
Defines the process of systematically monitoring animals after a procedure or when illness is expected, to reduce non-experimental variation caused by animal pain or distress.
What features are best interpreted as indicators of pain or distress in mice and rats?Rodents. Pain in rodents usually results in decreased activity, piloerection and an un-groomed appearance, or there may be excessive licking and scratching. They may adopt an abnormal stance with ataxia, but rats and mice may become unusually aggressive when handled. Acute pain may cause vocalization.
What normal behavior may mask whether a rat is experiencing pain or distress?Rats and Mice*
Rodents in pain generally have decreased activity, but increased activity may also be seen. Other abnormal behaviors include back-arching, belly-pressing, twitching, and staggering.
Which of the following is a strong indication for pain or distress in a mouse?Body Weight Changes. Reductions in body weight and growth rate are commonly used as indicators of pain and distress and as humane endpoints in research rodent studies.
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